A youthful looking Reading FC side tasted defeat in the EFL Trophy away to Cheltenham Town on Tuesday night.
Ruben Selles made wholesale changes to his team with an average age of just 19 in the starting 10 outfield players.
There were early chances for Reading as Emmanuel Osho came close just two minutes in when he forced Day into making a stop.
The hosts went closer to opening the scoring in the first-half but were unable to breach David Button in the Reading net as the teams went in level at the halfway stage.
Winger Adrian Akande created a big chance for himself when he skipped last a defender but was thwarted by the keeper.
Button made a close range save to keep it goalless before Wellens tested the Cheltenham keeper with a free-kick.
The Robins broke the deadlock with just over 10 minutes to go when Tom Pett smashed in a volley.
The result puts Reading in 2nd spot in Group P having one win and lost one of their two EFL Trophy matches.
The Royals are back in League One action on Saturday away to 15th placed Stevenage.
Cheltenham Town: Day, Taylor, Norkett, Bowman, Dulson, Bakare, Sohna, Shipley, Willcox, Pett, Liggett
Subs: Diallo, Jude-Boyd, Thomas, Colwill, Payne, King, Tustin
Reading FC: Button, Rushesha, Tuma, Holzman, Akande, Stickland, Ahmed, Sackey, Spencer, Osho, Barough
Subs: Boyce-Clark, Senga, Wellens, Beadcroft, Okine-Peters, Borgnis, Duah
